Table 1.

Summary of features and PCR results for nine cases of elephant endotheliotropic herpesvirus (EEHV) disease in Asian elephants (Elephas maximus) from India.

Case number	Place	PCR loci subtypes or polymorphisma						Key features or commentsb
		vGPCR	U71/gM	POL	TER	TK/gH	HEL
IP01	Muthanga	A2	Δ++, 289C	A, 960C	A1	A1	603T,636A, 774G, 825T, 930G	“Sunni”, M, 12 yo, 2005, captive born
IP05	Thekkady	E	ΔΔΔ,289C,562T	—	B2/A	F	764T	Wild, M, 5 yo, 2007
IP06c	Kodanad-1	E	+++, 289T	A	A2,211T	C3	603T,636A	“Nirangen”, M, 2 yo, 2007, rescued wild born
IP07c	Kodanad-2	E	+++, 289T	A	A2,211T	C3	603T,636A	“Aswathi”, F, 2 yo, 2007, rescued wild born
IP11	Pathiri-1	E/A	Δ++, 289C	A, 960C	A1	E	36A,774G,825T,930G	Wild, F, 6 yo, 2008
IP43	Vandalur	D1	Δ++, 289C	—	—	C1	603T,636A,774G,825T	“Chellama”, F, 8 yo, 2009, rescued wild born
IP60	Thrissur-1	D2/A	+++, 88T,442C	—	—	D**	774G,825T,930G	Wild, F, 8 yo, 2010
IP91	Thirunelli	A2	Δ++, 289C	A, 960C		D*	858A	Wild, M, 18 m, 2011
IP93	Thrissur-2	B1	B	B	B1	—	—	M, 7 yo, 2011, captive-born (Andaman Island)

^aEither overall gene subtype assignments (A, B, C1, C3, D*, D**, E/A, etc.) or the positions of minor strain polymorphism variability are indicated for each of the six PCR loci analyzed. For the U71/gM and HEL PCR loci, all data=A subtype if B is not stated. Polymorphisms are defined by changes from the consensus nucleotide at numbered standard nucleotide positions within each PCR locus. Bold type indicates unique polymorphisms found only in India. — = not done; +/Δ = presence/absence of 3× inserted/deleted codon triplets.

^bWord in quotations is given name of elephant; M = male; F = female; yo = years old; year = year sample taken.

^cThese two cases had identical DNA sequences at all PCR loci tested, including in multiple tissue samples examined from each. Two DNA preparations from each were also found to be identical for cases IP05, IP11, IP43, and IP60.