Table 1.
ACR adducted peptides identified using shotgun proteomics in rat N27 dopaminergic cells. There were 103 unique peptides identified that contained an ACR adduct. Adducted cysteine (Cys) groups are indicated with a small “c” in the peptide sequence. Note that a group of proteins have multiple Cys-containing peptides that are susceptible to ACR adduct formation and that some peptides containing multiple Cys groups are adducted. Complete information on the peptides and localization of adducted Cys groups are reported in Appendix 1.
| Major Cell function /Process |
Protein name | Protein accession numbers |
Protein molecular weight (Da) |
Peptide sequence |
|---|---|---|---|---|
| Cellular Metabolism/Mitochondria | Adrenodoxin, mitochondrial | ADX_RAT | 20116.8 | RLLVcGTR |
| Aspartyl-tRNA synthetase, cytoplasmic | SYDC_RAT | 57108.7 | QMcICADFEK | |
| ATP synthase subunit b, mitochondrial | AT5F1_RAT | 28851.5 | cIGDLK | |
| ATP-binding cassette sub-family A member 7 | ABCA7_RAT | 237705 | VAMVASGSLcCCGSPLFLRR | |
| Mitochondrial import inner membrane translocase subunit Tim13 | TIM13_MOUSE | 10440.1 | cIAMCMDR | |
| Triosephosphate isomerase | TPIS_RAT | 26830.7 | cLGELICTLNAAK | |
| Cell Signalling | Calcium/calmodulin-dependent protein kinase type 1 | KCC1A_RAT | 41621.6 | DCcVEPGSELPPAPPPSSR |
| COP9 signalosome complex subunit 1 | CSN1_RAT | 53411.8 | HVINMcLNVIK | |
| GTPase Hras | RASH_RAT | 21296.2 | LNPPDESGLGcMScK | |
| GTPase IMAP family member 4 | GIMA4_RAT | 35805.6 | YcLFNNK | |
| GTPase IMAP family member 8 | GIMA8_RAT | 77054.9 | GRVcAFNNK | |
| Islet amyloid polypeptide | IAPP_RAT | 9996.7 | KcNTATCATQR | |
| Latent-transforming growth factor beta- binding protein 2 | LTBP2_RAT | 189841 | KVTNDVcSQPLR | |
| Low molecular weight phosphotyrosine protein phosphatase | PPAC_RAT | 18133.8 | cCKAFLEK | |
| Mitogen-activated protein kinase 1 | MK01_RAT | 41259.9 | KISPFEHQTYcQRTLR | |
| Phosphatidylinositol-4,5-bisphosphate 3- kinase catalytic subunit beta isoform | PK3CB_RAT | 122595 | AKGKEAMHTcLK | |
| Protein phosphatase 1 regulatory subunit 12A | MYPT1_RAT | 115268 | ScSFGR | |
| Protein strawberry notch homolog 1 | SBNO1_RAT | 140727 | FFKYLcIASKVK | |
| Rab GDP dissociation inhibitor beta | GDIB_MOUSE | 50521.1 | TDDYLDQPCcETINR | |
| Rab proteins geranylgeranyltransferase component A 1 | RAE1_RAT | 72462.2 | KFLQcLGR | |
| Ras GTPase-activating protein SynGAP | SYGP1_RAT | 144708 | GKGGcPAVR | |
| Serine/threonine-protein kinase TAO3 | TAOK3_RAT | 105458 | GcYLK | |
| TANK-binding kinase 1-binding protein 1 | TBKB1_RAT | 67137.4 | SPASPScPSPVPQRR | |
| Type II inositol-3,4-bisphosphate 4- phosphatase | INP4B_RAT | 105230 | KLNGIRFTccK | |
| Vascular endothelial growth factor D | VEGFD_RAT | 37093.6 | cLPTGPRHPYSIIR | |
| Cell Structure | Alpha-1-acid glycoprotein | A1AG_RAT | 23557.8 | cSEQQKQQLELEK |
| Annexin A1 | ANXA1_RAT | 38813 | GDRcEDMSVNQDLADTDAR | |
| Annexin A1 | ANXA1_RAT | 38813 | cEDMSVNQDLADTDAR | |
| Collagen alpha-1(III) chain | CO3A1_RAT | 138919 | NcRDLKFCHPELK | |
| Collagen alpha-1(XXVII) chain | CORA1_RAT | 187795 | VcRDLMDcEQK | |
| Contactin-associated protein like 5–3 | CTP5C_RAT | 145704 | ANSLGFIGcLSSVQYNHIAPLK | |
| Cullin-associated NEDD8-dissociated protein 2 | CAND2_RAT | 139659 | TLIQcLGSVGRQAGHR | |
| Cysteine desulfurase, mitochondrial | NFS1_RAT | 49995 | cVLDScR | |
| Echinoderm microtubule-associated protein-like 2 | EMAL2_RAT | 70690.8 | ITQEVLGAHDGGVFALcALR | |
| Integrin alpha-7 | ITA7_RAT | 124178 | NVTLDcAQGTAK | |
| Intercellular adhesion molecule 1 | ICAM1_RAT | 60124.2 | cRAFSSR | |
| Tubulin beta-7 chain | TBB7_CHICK | 49652.6 | TAVcDIPPR | |
| Ion Channel and Receptors | 5-hydroxytryptamine receptor 2A | 5HT2A_RAT | 52833.4 | SLQKEATLcVSDLSTRAK |
| Glutamate receptor, ionotropic kainate 3 | GRIK3_RAT | 104057 | NcNLTQIGGLIDSK | |
| Inositol 1,4,5-trisphosphate receptor type 2 | ITPR2_RAT | 307044 | cGAFMSKLINHTKK | |
| Low-density lipoprotein receptor-related protein 2 | LRP2_RAT | 519241 | TCSAGEFScANGRCVR | |
| Melanocortin receptor 3 | MC3R_RAT | 35848.8 | IAALPPADGVAPQQHScMK | |
| Nuclear receptor subfamily 2 group C member 2 | NR2C2_RAT | 65327.8 | cLEMGMK | |
| Potassium voltage-gated channel subfamily H member 6 | KCNH6_RAT | 105691 | QDLDcWHR | |
| Tyrosine-protein phosphatase non-receptor type 11 | PTN11_RAT | 68440 | cNNSKPK | |
| Voltage-dependent anion-selective channel protein 3 | VDAC3_RAT | 30780.8 | ScSGVEFSTSGHAYTDTGK | |
| Voltage-dependent calcium channel subunit alpha-2/delta-3 | CA2D3_RAT | 122190 | cERLKAQK | |
| Neuronal Function | Fragile X mental retardation protein 1 homolog | FMR1_RAT | 66762.6 | QMcAK |
| Glial cell line-derived neurotrophic factor | GDNF_RAT | 23601.7 | YCSGScEAAETMYDK | |
| Huntingtin-associated protein 1 | HAP1_RAT | 70195 | KFITDPAYFMERcDTR | |
| Mesencephalic astrocyte-derived neurotrophic factor | MANF_RAT | 20370.9 | KDSQIcELKYDK | |
| N-acylneuraminate cytidylyltransferase | NEUA_RAT | 48111.6 | RVGLSAVPADAcSR | |
| Neurogenic locus notch homolog protein 2 | NOTC2_RAT | 265341 | VASFSCLcPEGK | |
| Protein Catabolism | E3 ubiquitin-protein ligase RNF123 | RN123_RAT | 149063 | VcATCFDLSVSLLR |
| Ubiquilin-like protein | UBQLN_RAT | 67775.8 | cQMEQLVLVSMGR | |
| Proteins with Domains | FACT complex subunit SSRP1 | SSRP1_RAT | 80899.7 | ELQcLTPR |
| F-box/LRR-repeat protein 20 | FXL20_RAT | 30443.2 | LRHLDLAScTSITNMSLK | |
| FERM domain-containing protein 8 | FRMD8_RAT | 51763.4 | QGSVVcSR | |
| Homeobox protein unc-4 homolog | UNC4_RAT | 53861.9 | ATPANTAATAGLDFTPGLPcAPR | |
| LIM domain kinase 1 | LIMK1_RAT | 72576.2 | cLEHPNVLK | |
| RIMS-binding protein 2 | RIMB2_RAT | 115598 | LNTAAcTYR | |
| Spermatogenic leucine zipper protein 1 | SPZ1_RAT | 45797 | cPNDCLTK | |
| WAP four-disulfide core domain protein 1 | WFDC1_RAT | 23211.9 | CPPPPRTLPPGAcQATR | |
| Zinc finger protein 574 | ZN574_RAT | 99334.3 | cLLcSR | |
| Response to Stress/Xenobiotics | Metallothionein-1; Short=MT-1 | MT1_RAT | 5987.3 | KSCcSCCPVGCSKCAQGCVCK |
| Peroxiredoxin-1 | PRDX1_RAT | 22092.2 | HGEVcPAGWKPGSDTIKPDVNK | |
| Transcription/Translation | 40S ribosomal protein S2 | RS2_RAT | 31214.2 | cSKEVATAIR |
| 60S ribosomal protein L11 | RL11_CHILA | 20235.2 | NEKIAVHcTVRGAK | |
| 60S ribosomal protein L12 | RL12_RAT | 17828.1 | cTGGEVGATSALAPK | |
| DNA polymerase delta catalytic subunit | DPOD1_RAT | 123586 | LALTLRPcAPILGAK | |
| Elongation factor 1-gamma | EF1G_RAT | 50043.4 | AAAPAPEEEMDEcEQALAAEPK | |
| Eukaryotic translation initiation factor 5B | IF2P_RAT | 137673 | RLAHcEELR | |
| Threonyl-tRNA synthetase, mitochondrial | SYTM_RAT | 81655 | LLGVLAEScGGR | |
| Transcriptional adapter 2-alpha | TAD2A_RAT | 51382.7 | TKEEcEK | |
| Various Functions | Autophagy-related protein 7 | ATG7_RAT | 77419.7 | cLLLGAGTLGCNVARTLMGWGVR |
| Carbonyl reductase family member 4 | CBR4_RAT | 25268.8 | VcAVFGGSR | |
| Chromodomain-helicase-DNA-binding | CHD8_RAT | 290678 | cKKNNK | |
| protein 8 | ||||
| DDB1- and CUL4-associated factor 8 | DCAF8_RAT | 66137 | FLPNSGDSTLAMcAR | |
| Embryonic polyadenylate-binding protein 2 | EPAB2_RAT | 29843.6 | ELLSPETIGcFFPGAPK | |
| Epithelial splicing regulatory protein 1 | ESRP1_RAT | 75003.8 | FFKGLNIAKGGAALcLNAQGR | |
| Intestinal mucin-like protein | MUC2L_RAT | 91478.1 | cTFFSCMK | |
| Kinesin-like protein KIF27 | KIF27_RAT | 158864 | SIAQLQGVKPVKVcR | |
| Lanosterol synthase | ERG7_RAT | 83284 | GIRcLLGK | |
| Lupus La protein homolog | LA_RAT | 47761.7 | MGcLLK | |
| Mast cell carboxypeptidase A | CBPA3_RAT | 47927.8 | KAIFMDcGIHAR | |
| Max-interacting protein 1 | MXI1_RAT | 26004.5 | RAHLRLcLER | |
| Pleckstrin homology domain-containing family M member 1 | PKHM1_RAT | 117492 | KGcPRCAR | |
| Pleckstrin homology domain-containing family M member 1 | PKHM1_RAT | 117492 | GKSWISEDDFcRPPK | |
| Protein hairless | HAIR_RAT | 127289 | LCVAcGRIAGAGKNR | |
| Protein SEC13 homolog | SEC13_RAT | 35528.8 | KFASGGcDNLIK | |
| Protein YIPF4 | YIPF4_RAT | 27266.5 | IRcVLMPMPSLGFNRQVVR | |
| Ribonucleoside-diphosphate reductase subunit M2 | RIR2_RAT | 45022.1 | EYLFNAIETMPcVKK | |
| SCO-spondin | SSPO_RAT | 550599 | cANGDcALK | |
| Selenoprotein P | SEPP1_RAT | 43064.7 | IAYcEKR | |
| Solute carrier family 2, facilitated glucose transporter member 5 | GTR5_RAT | 55527.3 | GALLFNNIFSILPAILMGcSK | |
| Spindle and kinetochore-associated protein 2 | SKA2_RAT | 16499.3 | ScICAILNK | |
| Taperin | TPRN_RAT | 80254.2 | EGGcPRPAISDTDK | |
| Thrombospondin-4 | TSP4_RAT | 108196 | GDAcDDDMDGDGIK | |
| Thrombospondin-4 | TSP4_RAT | 108196 | cGPCKPGYTGDQTR | |
| Torsin-2A | TOR2A_RAT | 35853.2 | SWVQGNLTAcGR | |
| Transmembrane protein 199 | TM199_RAT | 23142.6 | NVTcQDAQcGGTLSDLGK | |
| Uncharacterized protein C18orf54 homolog | CR054_RAT | 40829 | KcGDKIELLILK | |
| Vacuolar protein sorting-associated protein 54 | VPS54_RAT | 108826 | cKNIcPPK |