CsCyp is a member of the divergent Cyp subgroup. A, Protein sequence alignment of CsCyp with Arabidopsis ROC3 (AtROC3), wheat CypA-1 (TaCypA-1), C. elegans Cyp3 (CeCyp3), human CypA (HsCypA), and yeast (Saccharomyces cerevisiae) Cpr1 (ScCpr1) performed with ClustalW. Arrows indicate the amino acid residues that are part of the active site of CsCyp and that bind CsA. Residues comprising the divergent loop are shown in boldface underlined, whereas the conserved Cys residues (Cys-40 and Cys-168) and Glu (Glu-83) are shown in boldface. B, Phylogenetic analysis of Cyp proteins from plants (monocots and dicots), humans, nematodes, and yeast, showing that CsCyp clusters with uncharacterized Cyp proteins from dicot plants. CsCyp is not related to Arabidopsis Cyp59. Only the PPIase domain of AtCyp59 was used in the alignment.