Figure 7.
Binding dynamics of CsCyp to the YSPSAP and FGPDLP peptides. A, Superposition of 1H-15N HSQC spectra of free CsCyp (light red) and CsCyp titrated with peptide YSPSAP (darkening shades of red). B, Superposition of 1H-15N HSQC spectra of free CsCyp (light red) and CsCyp titrated with peptide FGPDLP (darkening shades of red). In both spectra, the arrows indicate the directions of the secondary chemical shifts of the backbone amide nuclei of the amino acids on addition of peptide. The labeled amino acids belong to the active site. The insets show signals for some of the amino acids that undergo a chemical shift perturbation and that were used to calculate the Kd of the interactions.