Figure 7.

Effect on relative growth rate of altering the T6P/SnRK1 signaling pathway by transferring to 10°C for 24 h and through expression of otsB and KIN10. Seedlings were grown with 0.5% (w/v) Suc for 7 d at 22°C, either transferred to 10°C for 24 h (10°C) and then back to 22°C or held at 22°C throughout (22°C). All growth measurements were performed at 22°C. A, Relative growth rate of Columbia-0 (Col 0) wild type (WT) compared with otsB maintained at 22°C. B, Relative growth rate of Columbia-0 wild type compared with otsB transferred from 10°C. C, T6P contents of the wild type and otsB upon transfer from 10°C and control maintained at 22°C. D, Suc contents of the wild type and otsB upon transfer from 10°C and control maintained at 22°C. E, Relative growth rate of AKIN10 compared with KIN10 maintained at 22°C. F, Relative growth rate of KIN10 compared with AKIN10. The data are means with se of three independent samples. (Note: T6P levels of otsB control were below detection limit.)