Figure 4.

Kernel phenotypes of zein RNAi lines. A, Half kernels showing vitreous or opaque endosperm. B, Kernels on a light box showing vitreousness or opacity. C, RNAi transgenes present in A, B, D, and E. D, Embryo genomic PCR showing genotype of kernels shown in E, which were used for TEM analysis. PCRs for double and triple construct lines were conducted separately and combined for agarose gel electrophoresis. Minus symbol refers to nontransgenic HiII control kernel, and plus symbol refers to the transgenic kernel. Size for PCR product in base pair is indicated. E, SDS-PAGE of purified zeins from developing endosperms of genotypes shown in D.