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Schematic overview of ABA-induced signaling by PYR/RCAR receptors leading to sequestration of PP2Cs, activation of protein kinases OST1, CPK21, and CPK23, and subsequent phosphorylation/activation of the SLAC1 anion channel that is essential for anion efflux and stomatal closure. Mutants selected for this study are shown. Detailed descriptions of the mutants are provided in Table I. [See online article for color version of this figure.]
