Table I. Descriptions of the mutants used in the study.
EMS, Ethyl methanesulfonate; T-DNA, transfer DNA.
| Genotype | Mutation | Description | Reference |
|---|---|---|---|
| ABA biosynthesis and catabolism | |||
| aba3-1 (Col-0) | EMS, G to A at position 3,707 | Defective in the conversion of ABA-aldehyde to ABA, ABA deficient | Léon-Kloosterziel et al. (1996); Nambara and Marion-Poll (2005) |
| aba1-1 (Ler) | EMS, G to A at position 2,139 (premature stop codon) | Defective in the ABA biosynthetic enzyme zeaxanthin epoxidase, strongly ABA deficient | Rock and Zeevaart (1991); Nambara and Marion-Poll (2005) |
| cyp707a1 (Col-0) | SALK_069127 | Defective in ABA 8′-hydroxylase, responsible for ABA catabolism in guard cells | Okamoto et al. (2009) |
| cyp707a3 (Col-0) | SALK_078173 | Defective in ABA 8′-hydroxylase, responsible for ABA catabolism in vascular tissues | Okamoto et al. (2009) |
| Core ABA signaling | |||
| abi1-1C (Col-0) | EMS, Gly-180 to Asp | Dominant point mutation in ABI1 resulting in the loss of PYR/RCAR binding and ABA insensitivity | Nishimura et al. (2004); Umezawa et al. (2009) |
| abi1-1L (Ler) | EMS, Gly-180 to Asp | See abi1-1C | Leung et al. (1997); Ma et al. (2009) |
| abi2-1C (Col-0) | EMS, Gly-168 to Asp | Dominant point mutation in ABI2, resulting in the loss of PYR/RCAR binding and ABA insensitivity | Nishimura et al. (2004) |
| abi2-1L (Ler) | EMS, Gly-168 to Asp | See abi2-1C | Leung et al. (1997); Ma et al. (2009) |
| abi1-2 hab1-1 pp2ca-1 (Col-0) | SALK_072009, SALK_002104, SALK_028132 | Triple knockout mutant of PP2Cs ABI1, HAB1, and PP2CA | Rubio et al. (2009) |
| hab1G246D (Col-0) | Transgenic line | Overexpression of HAB1 carrying the G246D mutation that prevents the binding to PYR/PYL and ABA insensitivity | Robert et al. (2006); Dupeux et al. (2011) |
| ost1-2 (Ler) | EMS, G to A at position 97 | Point mutation in ABA-activated protein kinase OST1 | Mustilli et al. (2002) |
| ost1-3 (Col-0) | SALK_008068 | = srk2e = snrk2.6, T-DNA knockout mutation of ABA-activated protein kinase OST1 | Yoshida et al. (2002) |
| pyr1pyl1pyl4 (114) (Col-0) | EMS + T-DNA | Triple mutant of ABA receptor proteins | Park et al. (2009) |
| pyr1pyl4pyl5 (145) (Col-0) | EMS + T-DNA + transposon | Triple mutant of ABA receptor proteins | Gonzalez-Guzman et al. (2012) |
| pyr1pyl4pyl8 (148) (Col-0) | EMS + T-DNA | Triple mutant of ABA receptor proteins | Gonzalez-Guzman et al. (2012) |
| pyl4pyl5pyl8 (458) (Col-0) | T-DNA + transposon | Triple mutant of ABA receptor proteins | Gonzalez-Guzman et al. (2012) |
| pyr1pyl1pyl2pyl4(1124C) (Col-0) | EMS + T-DNA | Quadruple mutant of ABA receptor proteins | Park et al. (2009) |
| pyr1pyl1pyl2pyl4 (1124L) (Ler) | EMS + T-DNA | Quadruple mutant of ABA receptor PYR/PYL/RCAR proteins | Park et al. (2009) |
| pyr1pyl4pyl5pyl8(1458) (Col-0) | EMS + T-DNA + transposon | Quadruple mutant of ABA receptor proteins | Gonzalez-Guzman et al. (2012) |
| pyr1pyl2pyl4pyl5pyl8(12458) (Col-0) | EMS + T-DNA + transposon | Pentuple mutant of ABA receptor proteins | Gonzalez-Guzman et al. (2012) |
| pyr1pyl1pyl2pyl4pyl5pyl8 (112458) (Col-0) | EMS + T-DNA + transposon | Sextuple mutant of ABA receptor proteins | Gonzalez-Guzman et al. (2012) |
| Other mutants | |||
| cpk21 (Col-0) | GABI_322A03 | Defective in CPK21 | This study (Supplemental Fig. S9) |
| cpk23 (Col-0) | SALK_007958 | Defective in CPK23 | Geiger et al. (2010) |
| slac1-3 (Col-0) | SALK_099139 | T-DNA insertion in SLAC1 | Vahisalu et al. (2008) |
| slac1-7 (Col-0) | C to T at position 527 | Point mutation of Ser-120 to Phe in SLAC1 | Vahisalu et al. (2010) |