Figure 2.

Cys-20 of the TCP domain is required for redox sensitivity. A, The redox sensitivity of TCP16, which contains Val instead of Cys at position 20 of the TCP domain, was analyzed as described in Figure 1. B, A similar experiment as in A with a TCP15 mutant protein that contains Ser instead of Cys-20. The amount of bound DNA, relative to the sample incubated with DTT, is shown below the EMSA images. Experiments were repeated at least three times with similar results.