Figure 3.

Oxidation of TCP proteins that contain Cys-20 leads to the formation of intermolecular bonds. A and B, TCP proteins (1 µg of MBP-TCP fusion) that either contain (A) or lack (B) Cys-20 within the TCP domain were incubated with redox agents as described in Figure 1. C, Purified MBP was subjected to a similar treatment. After treatment, the molecular mass of the proteins was analyzed in a nonreducing SDS-PAGE. Arrowheads point to species with the mobility of monomers (M) or dimers (D). MW, Molecular mass markers. Experiments were repeated at least three times with similar results.