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TCP15 is inactivated by modification of Cys-20. TCP15 and C20S-TCP15 were incubated with 2.5 or 5 mm NEM in the dark during 20 min at 25°C. After treatment, the DNA-binding activity of the proteins was analyzed by EMSA. The amount of bound DNA, relative to the untreated sample (–), is shown below the EMSA images. The experiment was repeated at least three times with similar results.
