Figure 9.

Modulation of CUC1 expression by class I TCP proteins is affected by H₂O₂ treatment. An Arabidopsis GUS reporter line for CUC1 expression was transformed with constructs that express fusions of class I TCP proteins to the EAR repressor domain under the control of the 35S CaMV promoter or with constructs that express the HD-Zip transcription factor Hahb4 (Dezar et al., 2005) or GUS as a control. Twenty-four hours after transformation, plants were treated with either water (Control) or 4 mm H₂O₂ during 12 h and then used to analyze GUS expression by histochemistry. About 40 transformed plants were analyzed for each condition. Representative images of the patterns obtained most frequently in each case are shown. A quantitative evaluation of the data are shown in Table I. The experiment was repeated twice with similar results.