Figure 1.

DNA-binding specificity and transcriptional activation activity of GhTCP14. A, Gel-shift assay. GhTCP14 proteins were incubated with biotin-labeled probes (class I probe: 2×TGGGTCCCACAT and class II probe: 2×TTGTGGGCCCCT) in the presence or absence of 20-fold excess of nonlabeled competitor. Arrow indicates the position of protein-DNA complexes. B, Transcriptional activation ability of GhTCP14 in Arabidopsis protoplasts. The GAL4 DNA-binding domain (BD) and BD-VP16 were used as negative or positive control, respectively. Error bars represent the sd of three biological replicates.