Figure 4.

Relative growth of C. polonica isolates 1 and 2 in artificial medium amended with astringin in relation to the rates of phenolic biotransformation and the ability to utilize phenolics as sole carbon sources. A, Biotransformation. Depletion of astringin added to artificial medium over a period of 72 h was compared with changes in astringin concentration in sterile control medium (n = 4). B, Growth. In vitro growth on solid minimal medium was determined in the presence of astringin relative to growth without astringin (n = 10). Error bars in A and B represent se. C to F. Utilization. Changes in the ratio of ¹³CO₂ to ¹²CO₂ were determined in fungal head space arising from respiration of [U-¹³C]caffeic acid. Samples were collected over 18 d of fungal growth on agar containing 1 mm caffeic acid amended with 10 µm (C), 2 µm (D), 0.4 µm (E), and 0.08 µm (F) [U-¹³C]caffeic acid (n = 1). VPDB, Vienna Pee Dee Belemnite. [See online article for color version of this figure.]