Figure 1.

Phylogeny and conservation of TX and TN protein domains. A neighbor-joining phylogenetic tree shows the homology of TN sequences across plants. Arabidopsis TN proteins AtTN21 and AtTN17 (highlighted in color) were used as template sequences to compare against plant protein databases. The evolutionary history was inferred using the neighbor-joining method (Saitou and Nei, 1987). The bootstrap consensus tree inferred from 1,000 replicates is taken to represent the evolutionary history of the taxa analyzed. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates are collapsed. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. The analysis involved 50 amino acid sequences. All ambiguous positions were removed for each sequence pair. There were a total of 1,556 positions in the final data set. Evolutionary analyses were conducted in MEGA5 (Tamura et al., 2011).