Figure 3.

Transient overexpression of Arabidopsis TN and TX genes in tobacco results in partial cell death response. A, Transient overexpression of AtTN3 in tobacco results in cell death. Visual scoring of HR responses and HR strength score table (++++ = 4; +++ = 3; ++ = 2; and + = 1) indicate the strength of HR observed with the transient overexpression of other TX and TN genes compared with the TN gene AtTN3. B, Transient overexpression of AtTN3 along with elicitors SA, ethephon, and flagellin results in increased cell death responses in tobacco. C, Transient overexpression of AtTN3 in an eds1-silenced plant along with or without flagellin resulted in a decreased HR in tobacco. Expression of EDS1 was checked in tobacco plants in both empty vector transformed lines (EV) and EDS1-silenced lines (SL) by quantitative RT-PCR.