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. 2013 Jul 2;8(7):e67461. doi: 10.1371/journal.pone.0067461

Figure 3. Functional assays on miRNA-1 as a potential tumor-suppressor gene.

Figure 3

(A) AlamarBlue cell viability assay to test the effect of miRNA-1. SW480 (primary colon cancer cell line) and SW620 (corresponding metastases cell line) cells were transfected with miRNA-1 mimics (+miR-1) or mock transfected (−miR-1) and measured using an spectrophotometer after 24 h, 48 h and 72 h. The miRNA-1 level was determined by TaqMan assays for mature microRNAs. (B) “Wound healing” assay for miRNA-1 in SW480 and SW620 cells. After 24 h of transfection with miRNA-1 mimics a uniform scratch was generated through each confluent cell layer and “wound” closure was documented after 24 h using a phase-contrast microscope (n = 2). (C) AlamarBlue cell viability assay in SW480 and SW620 cells after camptothecin treatment alone or in combination with miRNA-1. Cell viability was measured after 0 h, 24 h and 48 h of drug treatment using a spectrophotometer.