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. 2013 Apr 3;33(14):6181–6190. doi: 10.1523/JNEUROSCI.4491-12.2013

Figure 2.

Figure 2.

Human recombinant 3K3A-APC increases migration of human NPCs. A, Migration of NPCs determined in a Boyden chamber 5 h after plating with or without human 3K3A-APC, bFGF, or S360A-APC at 1.4 nm. The migration index represents the number of migrated cells based on relative fluorescence units. B, Representative neurospheres labeled with Cell Tracker Green CMFDA 16 h after plating on poly-l-ornithine-coated surface (top) or in 3D Matrigel (bottom) with vehicle, 3K3A-APC, or S360A-APC at 1.4 nm. Solid circle denotes the neurospheres; arrow shows the leading edge of migrating cells. Scale bar, 100 μm. (C–D) Quantification of neurosphere migration cultured on poly-l-ornithine surface as in B (top) showing the size of neurospheres (Φ: diameter; C) and the migration index (D). E, F, Quantification of neurospheres cultured in 3D Matrigel as in B (bottom) showing the size of neurospheres (Φ: diameter; E) and the migration index (F). In A and C–F, data are shown as mean ± SEM, n = 3 independent cultures in triplicate. In C and E, the control neurosphere diameter was arbitrarily taken as 100%. Statistical significance was determined by one-way ANOVA followed by Tukey's post hoc test. NS indicates not significant.