Fig. 2.

Effect of chemical inhibitors on IFN-γ production by human NK cells. Enriched NK cells were stimulated with 100 ng/mL IL-18 alone (darker solid bars), immobilized IgG alone (lighter solid bars), or both agents (striped bars) in the absence of chemical inhibitors (“Control”) or in the presence of 10 μM Wortmannin, U0126, or SB203580 as indicated. P value for comparison of control versus inhibitor is <0.0001 for each inhibitor when comparing IFN-γ levels produced by NK cell stimulated with IgG alone or IgG plus IL-18. Results shown represent mean ± SE of data from 5 separate experiments