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Dendritic spines on pre-BötC excitatory inspiratory neuron. Two-photon, live Z-projection image of pre-BötC glutamatergic intrinsic burster neuron in vitro filled with Alexa 594 dye. Proximal dendrites exhibit numerous spine-like structures. Higher resolution scan image of dendritic segment (bottom left, inset, from dashed box in lower magnification image) shows examples of spines: filament-type (arrowheads) and mushroom-type (arrow pointing to spine head).
