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. 2013 Feb 13;33(7):2994–3009. doi: 10.1523/JNEUROSCI.4427-12.2013

Figure 3.

Figure 3.

Identification of neurotransmitter phenotype of pre-BötC inspiratory neurons by single-cell multiplex RT-PCR. Electrophoresis gel generated by multiplex RT-PCR from mRNA in cytoplasm aspirated from 5 representative pre-BötC inspiratory neurons showing cDNA for VgluT2 expression by intrinsic burster neuron (lane 2), VgluT2 by intrinsic nonburster neuron (lane 3), GlyT2 (lane 4), GAD67 (lane 5), and both GlyT2 and GAD67 (lane 6), respectively, expressed by intrinsic nonburster neurons. The positive control (lane 1) from 100 pg of total rat brain RNA (tRNA) shows the predicted base pair (bp) molecular weights of amplimers of VgluT2 (315 bp), GlyT2 (701 bp), and GAD67 (185 bp). No VgluT2, GlyT2, or GAD67 signals were present in negative controls (lane 7) from the “mock harvests.”