Generation of MpCAO overexpression of Arabidopsis lines.
A, phenotypes of wild-type and transgenic lines. 1, wild type; 2, MpCAO1+MpCAO2–1; 3, MpCAO1+MpCAO2–2; 4, ch1-1; 5, MpCAO1; and 6, MpCAO2. B, confirmation of transgenic lines by PCR. C, confirmation of transgenic lines by immunoblot analysis. The MpCAO1-FLAG and MpCAO2-HA proteins were detected using anti-FLAG or anti-HA antibodies, respectively. Lane 1, wild type; lane 2, ch1-1; lane 3, MpCAO1; lane 4, MpCAO2; lane 5, MpCAO1+MpCAO2–1; lane 6, MpCAO1+MpCAO2–2.