TABLE 3.

Affinities of barbiturates for GABA_AR anesthetic-binding sites at the β⁺−α⁻ (R-³Hazietomidate) and α⁺/γ⁺−β⁻ (R³HmTFD-MPAB) subunit interfaces^a

^a IC₅₀ values, the total anesthetic concentrations resulting in 50% inhibition of GABA_AR photolabeling, were determined as described under “Experimental Procedures; EC₅₀ for anesthesia indicates tadpole loss of righting reflex.

^b See Ref. 16.

^c See Ref. 27.

^d See Ref. 55.

^e Data were calculated with ALOPS 2.1 program, VCCLAB, Virtual Computational Chemistry.