TABLE 5.
Pharmacological specificity of R-[3H]mTFD-MPAB photoincorporation into residues in the α1β3γ2 GABAAR (cpm/pmol of PTH-derivative)
The efficiency of photolabeling of a residue (in cpm/pmol) was calculated using Equation 5 (see under “Experimental Procedures”). The data are presented as mean (±range) when two samples were sequenced. Other values were determined from the sequencing of single samples. ND indicates not determined.
| Amino acid | Experiment 1 +GABA |
Experiment 2 |
|||
|---|---|---|---|---|---|
| Control | +Pentobarbital (1 mm) | Control | +GABA (1 mm) | +Etomidate (100 μm) | |
| β3M1 Met-227 | 950 ± 30 | 168 ± 5 | 420 | 640 ± 50 | 660 |
| α1M3 Ala-291 | 92 | <5 | 28 | 50 | 41 |
| α1M3 Tyr-294 | 56 | <5 | 36 | 46 | 26 |
| γ2M3 Ser-301 | 105 ± 5 | ND | 83 | 130 | 115 |
| β3M3 Met-286 | 25 | ND | 7 | 13 | <2 |
| β3M3 Phe-289 | 18 | ND | 12 | 10 | <2 |