FIGURE 2.

Immunocytochemical analysis of polytene chromosome morphology and H3K9 dimethylation in JIL-1 null larvae expressing JIL-1 transgenes. A, comparison of polytene chromosome squash preparations from wild type (wt) and JIL-1 null (z2/z2) third instar larval salivary glands labeled with JIL-1 antibody. B, polytene chromosome squash preparations from JIL-1 null (z2/z2) third instar larval salivary glands expressing the FL, the ΔCTD, the ΔNTD, and the CTD, respectively. Transgenic protein localization (in green) was identified using LacI antibody, H3K9 dimethylation (in red) was identified using H3K9me2 antibody, and DNA (in blue or gray) was labeled by Hoechst.