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. 2013 May 30;288(27):19441–19449. doi: 10.1074/jbc.M113.464271

FIGURE 4.

FIGURE 4.

Expression of transgenic JIL-1 kinase-dead constructs in a JIL-1 null background. A, diagrams of the JIL-1 V5-tagged constructs analyzed. B, immunoblot labeled with JIL-1 antibody of protein extracts from wild type (wt) and from JIL-1 null salivary glands expressing the KDI*/KDII*, KDII*, and KDI*, respectively. Labeling with tubulin antibody was used as a loading control. C, immunoblot labeled with H3S10ph antibody of protein extracts from salivary glands from wild type third instar larvae (wt) and from JIL-1 null salivary glands expressing KDI*/KDII*, KDII*, and KDI*, respectively. Labeling with histone H3 antibody was used as a loading control.