Expression of transgenic JIL-1 kinase-dead constructs in a JIL-1 null background.
A, diagrams of the JIL-1 V5-tagged constructs analyzed. B, immunoblot labeled with JIL-1 antibody of protein extracts from wild type (wt) and from JIL-1 null salivary glands expressing the KDI*/KDII*, KDII*, and KDI*, respectively. Labeling with tubulin antibody was used as a loading control. C, immunoblot labeled with H3S10ph antibody of protein extracts from salivary glands from wild type third instar larvae (wt) and from JIL-1 null salivary glands expressing KDI*/KDII*, KDII*, and KDI*, respectively. Labeling with histone H3 antibody was used as a loading control.