FIGURE 1.

Enhanced phosphorylation of GM-CSF receptor βc upon expression of CBL mutants. A, lysates from parental TF-1 cells as well as from TF-1 cells expressing wild type CBL, CBL-Y371H, and CBL-C384R were probed for CBL expression. IB, immunoblot. B, GM-CSFR βc immunoprecipitations (IP) were performed on lysates collected from TF-1 cells expressing CBL wild type, CBL-Y371H, and CBL-C384R mutants. The cells were stimulated with 0.5 ng/ml GM-CSF for the indicated times. Blots were probed with Tyr(P)- and GM-CSFR βc-specific antibodies.