FIGURE 3.

Expression of CBL-Y371H and CBL-C384R mutants results in elevated JAK2. A, lysates collected from TF-1 cells expressing wild type and CBL mutants were probed for phospho-Tyr-1007/1008 JAK2 and reprobed for total JAK2 and tubulin. B, blots were quantified using ImageJ software. Values presented are ratios of total JAK2 to tubulin, normalized to wild type CBL at time 0 (mean ± S.E. (error bars), n = 4; *, p ≤ 0.05). C, TF-1 cells were treated with proteasomal and lysosomal inhibitors, MG-132 and chloroquine (CQ), prior to stimulation (n = 4).