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Increased expression of LYN kinase in CBL-Y371H and CBL-C384R mutant TF-1 cells. A, whole cell lysates collected from TF-1 cells expressing CBL-WT and CBL mutants were immunoblotted with antibodies specific for Tyr(P)-396-LYN. Blots were reprobed for total LYN (p56) and tubulin. B, blots were quantified using ImageJ software. Values presented are ratios of total LYN to tubulin, normalized to CBL-WT at time 0 (mean ± S.E. (error bars), n = 6; *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001).
