Dasatinib treatment abolishes the enhanced phosphorylation of CBL-Y371H and CBL-C384R. TF-1 cells expressing WT and mutant CBL were incubated with dasatinib and TG101348 for 2 and 4 h, respectively, prior to GM-CSF stimulation. Cells were lysed, and HA immunoprecipitations were performed. Blots were probed with Tyr(P) antibodies and reprobed with HA-specific antibodies. IP, immunoprecipitation; IB, immunoblot.