FIGURE 7.

Inhibition of JAK2 activity via treatment with TG101348 abolishes the enhanced phosphorylation of GM-CSFR β in CBL mutant-expressing cells. TF-1 cells expressing WT-CBL, CBL-Y371H, and CBL-C384R were cytokine-depleted and treated with 2 mm TG101348 for 4 h or 0.1 mm dasatinib for 2 h prior to GM-CSF stimulation. GM-CSFRb-specific immunoprecipitations were performed on collected lysates. Immunoblotting was performed using Tyr(P)- and GM-CSFR βc-specific antibodies. IP, immunoprecipitation; IB, immunoblot.