TABLE 2.

Catalytic parameters of activation of ATFII and Elk-1 by p38α DEF site mutants

	GST-ATFll			GST-Elk-1
	Km	Kcat	Kcat/Km	Km	Kcat	Kcat/Km
	μm	min−1	min−1* μm−1	μm	min−1	min−1* μm−1
wt	119	100	0.84	472	6	0.013
Y258A	297	57	0.19	244	1	0.005
HI228–9AA	445	400	0.89	201	2	0.011
D361N	114	100	0.87	483	7	0.014