FIGURE 3.
Effects of EPI64 protein overexpression on the active state of endogenous Rab27B and Rab3D. Isolated mouse pancreatic acini were incubated with 2 × 107 pfu/ml adenoviruses coding for EPI64, EPI64B, or GFP, respectively, for 16 h. Acini were then lysed, and GST-SHD pulldown and GST-Rim pulldown were performed to examine the endogenous GTP-Rab27B and GTP-Rab3D levels. Anti-Rab27B and anti-Rab3D antibodies were used for Western blotting. Bars indicate means ± S.E. (error bars) from multiple independent experiments (A, n = 7; B, n = 4). *, p < 0.05; **, p < 0.01.