Overexpression of EPI64B enhances CCK and carbachol-stimulated amylase release in isolated ICR mouse pancreatic acini. Isolated acini were incubated with 2 × 107 pfu/ml adenoviruses coding for EPI64, EPI64B, or GFP alone for 16 h. Acini were then resuspended and incubated with various concentrations of CCK (A) or carbachol (B) for 30 min, and amylase release was determined. Results are mean ± S.E. (error bars) from multiple independent experiments (A, n = 6; B, n = 5). *, p < 0.05; **, p < 0.01.