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. 2013 May 13;288(27):19548–19557. doi: 10.1074/jbc.M113.472134

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — EPI64B physically interacts with Rab27B in vitro only when Rab27B is in the transition state. Isolated mouse pancreatic acini were incubated with 2 × 10⁷ pfu/ml adenovirus coding for HA-tagged EPI64B for 16 h. GST only or GST-Rab27B WT (Rab27B) beads were pretreated with GDP, GTPγS, or GDP/AlFx, respectively. The beads were then incubated with total lysates from acini overexpressing HA-tagged EPI64B. Western blotting of protein absorbed to the beads was performed using anti-HA antibody. The bottom panel shows the Coomassie staining of GST and GST-Rab27B proteins used in the pulldown.