FIGURE 1.

SDS-PAGE analysis of purified light chain. M, marker; 1, reduced condition; 2, non-reduced condition. The purified light chain sample (250 ng) was separated by 12% acrylamide gel electrophoresis and visualized by silver staining. Under the non-reduced condition, the monomeric (26-kDa) and dimeric (51- and 43-kDa) forms of 22F6 were observed. A weak band at 43 kDa may be a different dimeric form from that of the band at 51 kDa. Under the reduced condition, no other bands except for the 31 kDa band of the monomeric form were detected, indicating high purity.