TABLE 1.
Kinetic parameters for catalytic antibodies showing amidase (peptidase) and/or nuclease activity
| Name | Form | Substrate | Km | kcat | Reference/Source | Remarks |
|---|---|---|---|---|---|---|
| m | min−1 | |||||
| HIR (BJP) | L | Chromozyme TRY | 1.5 × 10−4 | 6.2 | Ref. 34 | |
| RHY | L | IEGR-MCA | 1.03 × 10−4 | 2.62 × 10−2 | Ref. 12 | |
| E6 | scFv | LAEEEV-MCA | 5.3 × 10−5 | 1.1 × 10−3 | Ref. 35 | |
| L12 | L | PFR-MCA | 5.3 × 10−5 | 1.55 × 10−3 | Ref. 35 | V = 0.03 μm/h (0.7 μm L) turnover = 0.04/h |
| 22F6 | L | QAR-MCA | Not measured | Not measured | This study | V = 0.09 μm/h (4 μm L) turnover = 0.02/h |
| 6B8-E12 | IgG | AAPFpNa | 1.4 × 10−3 | 1.5 | Ref. 36 | |
| 6B8-E12 | scFv | Leu-MCA | 5 × 10−5 | 0.52 | Ref. 36 | |
| YZ17 | Fv | EAR-MCA | 1.01 × 10−5 | 0.5 | Ref. 37 | |
| Hybrid IgG | IgGn | EAR-MCA | 1.546 × 10−4 | 1.77 × 10−2 | Ref. 38 | |
| BV 04-01 | scFv | plasmid DNA | 2.62 × 10−8 | 1.61 × 10−2 | Ref. 39 | |
| Human pIgG(2) | pIgG | scDNA | 7.6 × 10−5 | 1.5 × 10−3 | Ref. 9 | |
| Rabbit pAB | pAB | (pC)9 | 9 × 10−4 | 1.7 | Ref. 10 | |
| Rabbit pAB | pAB | (pU)9 | 2.9 × 10−3 | 1.0 | Ref. 10 | |
| 22F6 | L | pBR322 | 4.36 × 10−7 | 1.02 × 10−2 | This study |