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R36E/R37E is defective in enhancing cell viability in cancer cell lines under anchorage-independent conditions. Cells were plated in poly-HEMA-coated plates and incubated with 0, 1, 10, and 100 ng/ml WT IGF1 or R36E/R37E for 48 h. Cell viability was measured by MTS assays. Data are means ± S.E. (n = 6). Statistical analysis was performed using ANOVA and Tukey's multiple-comparison test.
