FIGURE 5.

TORC2-Gad8 mutants show synthetic sick interactions with components of the homologous recombination pathway. A and B, tetrad analysis of heterozygous diploids with the indicated mutations. Representative spores from five asci are shown for each cross. C, strains were grown to log phase in rich medium and visualized by light microscopy. Bar, 20 μm. D and E, 10-fold serial dilution of logarithmic growing cells were plated on YE-rich medium in the presence or absence of the indicated DNA-damaging agents and incubated at 30 °C for 3–4 days.