FIGURE 6.

Combinatorial effect of C. jejuni LOS sialylation, amide linkages, and phosphorylation on induction of TNFα in primary human monocytes. Primary monocytes were stimulated with 10 ng/ml LOS for 20 h, and TNFα was assessed by ELISA. TNFα levels were compared with the level of LOS sialylation (A), the relative abundance of four amide linkages (B), the relative abundance of three or four phosphoryl moieties in each strain (C), and a combination of relative sialylation, abundance of amide linkages, and phosphorylation (D). Data points represent mean values for an individual strain from five donors. A, one-way analysis of variance (*, p < 0.05). B, C, and D, linear regression analyses were performed.