TABLE 3.
PCR analysis reveals association of SA synthesis genes with livestock strains
| Non-livestock straina | NeuB1 | Class A/B | Class C | Livestock straina | NeuB1 | Class A/B | Class C |
|---|---|---|---|---|---|---|---|
| 33106 | + | + | − | 11168H | + | − | + |
| 33084 | + | + | − | 45557 | + | − | + |
| 40917 | + | − | + | 31485 | + | − | + |
| 31481 | − | − | − | 32799 | + | + | − |
| 56519 | − | − | − | 56282 | + | + | − |
| 32787 | − | − | − | 44811 | + | − | + |
| 64555 | − | − | − | 59161 | + | − | + |
| 43205 | − | − | − | 59214 | + | + | − |
| 44119 | − | − | − | 30280 | + | − | + |
| 63326 | + | + | − | 44958 | + | + | − |
| 62914 | − | − | − | 30328 | + | − | + |
| 45631 | − | − | − | 58473 | + | + | − |
| 59364 | − | − | − | 48612 | + | − | + |
| 52368 | − | − | − | ||||
| 56832 | + | + | − | ||||
| 38353 | − | − | − | ||||
| 12241 | + | + | − | ||||
| 53259 | + | + | − | ||||
| 35799 | + | + | − | ||||
| 62567 | + | − | + |
a PCR analysis used primers that anneal to neuB1 genes to identify strains that encode the SA biosynthesis pathway for human clinical isolates that clustered with either the non-livestock or livestock clades (21). Primers described previously (15) were used to distinguish strains from SA-positive LOS classes A/B from C.