FIGURE 7.

Compound in vivo redox analysis. A 384-well assay to determine whether reactive oxygen species is generated by compounds SW045263 (chemotype 1), SW025890 (chemotype 2), and SW155246 in A549 cells was performed. Compounds were titrated with 5000 cells/well. Carboxy-H₂DCFDA (50 μm) was used as an indicator and fluorescence was read at excitation 480 and emission of 520 4 h after addition of compound. Readings were normalized to no compound samples. Each point represents the average reading of triplicate wells. Conditions for all assays are described under “Experimental Procedures.”