TABLE 1.
Relative rates of PID binding to purified iNOSfl and consequent iNOS monomerization and inhibition of activity in the presence and absence of Arg and H4B
Rates were derived through linear fitting of the curves depicted in Fig. 3. Details of the experiment are given under “Experimental Procedures.”
Treatment | Rate of binding | Rate of monomerization | Rate of inhibition |
---|---|---|---|
min−1 | min−1 | min−1 | |
−Arg, −H4B | 1.862 ± 0.071 | 0.533 ± 0.014 | 0.913 ± 0.027 |
+Arg | 1.774 ± 0.043 | 0.471 ± 0.023 | 0.833 ± 0.014 |
+H4B | 1.272 ± 0.055 | 0.356 ± 0.011 | 0.654 ± 0.019 |
+Arg, +H4B | 1.111 ± 0.052 | 0.304 ± 0.005 | 0.511 ± 0.008 |