TABLE 2.

Primers used to verify the successful knock-out of genes in strains used in this study

Gene	Primer	Sequence (5′ → 3′)
asnB	U_asnB	GGATTCGAACCTGTGACCC
	D_asnB	CCAGCTGGATTTACCCGTC
	Internal asnB to U	GCAGCAGGTTTCTGATCAGC
	Internal asnB to D	GCTGATCAGAAACCTGCTGC
frmA	U_frmA	CCTGATCAACCATGATTTCCG
	D_frmA	CCAGTACTCCGGAAGAGAAG
	Internal frmA to U	CACGCATACCATGAGCCTG
	Internal frmA to D	CAAATGCAACGGCAGCACG
maeB	U_maeB	GTTAGCGTCATAATGCCAATTG
	D_maeB	CGAATTCCTTCCCAGGTCG
	Internal maeB to U	GATCAGGATCTGAGCTTTGG
	Internal maeB to D	CCAAAGCTCAGATCCTGATC
ridA	U_ridA	CGTTAAGCTATTTCCCGTGGC
	D_ridA	GCACGGTAATTGACCATATCC
	Internal ridA to U	GGTCAGATCCCGGTAAATCC
	Internal ridA to D	GGATTTACCGGGATCTGACC