FIGURE 5.

d-βOHB inhibits neonatal hepatic ketogenesis in vivo. Total βOHB pools (A), fractional ¹³C enrichments of βOHB (B), and ¹³C-βOHB concentrations (C) 20 min after intraperitoneal injection of sodium [1,2,3,4-¹³C₄]octanoate (10 μmol/g of body weight) alone or co-injected with [¹³C]octanoate plus 20 μmol/g of body weight of unlabeled AcAc, l-βOHB, or d-βOHB, in livers of milk-fed P0 mice. The [¹³C]octanoate alone datasets (the white bars in these panels) are reproduced from Fig. 3E for comparison. n = 5–7/group for each panel. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus wild-type neonates injected with [¹³C]octanoate alone, or as indicated by 1-way ANOVA. ††, p < 0.01; †††, p < 0.001 versus AcAc co-injected neonates. Error bars, S.E.