FIGURE 1.

[¹⁴C]Oleate and [³H]monoolein retention by WT and LFABP^−/− liver cytosol. Representative chromatograms of 10 mg of WT and LFABP^−/− cytosol (A₂₈₀; hollow symbols) and [¹⁴C]oleate (A and B) or [³H]monoolein (C and D) radioactivity (dpm; solid symbols) plotted against elution volume (ml) are shown. Separate columns were packed for the two [¹⁴C]oleate and the two [³H]monoolein fractionations. 50 μl of concentrated fraction volume was probed for LFABP in multiple fractions from each of the runs; LFABP immunoblots are shown in E–H. The fractions are labeled by their elution volumes, and the first lane in each immunoblot is the positive control 3 μg of purified LFABP.