FIGURE 1.

In silico screening for neuronal palmitoyl candidates. A–C, based on our criteria (≥1.8 CSS-Palm score and ≥0.75 brain enrichment score), we chose 573 proteins as brain-enriched palmitoyl candidates (supplemental Table S2). Note that secreted proteins and proteins with target cysteines only in extracellular regions were excluded. Of the candidate proteins, 84.6% have not yet been reported and are considered as promising new palmitoyl substrates (A). 35.7% of neuronal substrate candidates are membrane proteins (B). TMD, transmembrane domain. Our 12 selected candidates (red) and PSD-95 (blue), a representative neuronal palmitoyl protein, are marked on the scattergram, consisting of CSS-Palm scores (≥1.8) on the y axis and brain-enrichment scores (≥0.75) on the x axis (C).