TABLE 1.
Selected palmitoyl candidates in this study
Highest CSS-Palm score, Position, and Sequence indicate the highest score in the protein given by CSS-Palm 2.0, the position of the corresponding cysteine (underlined in sequence), and the sequence around the cysteine with amino acid positions, respectively. N (bold), N terminus; C (bold), C terminus. ND means no data retrieved from Body-Map database. “Metabolic” indicates the result of metabolic labeling assay with [3H]palmitate: +, palmitoylated; −, not palmitoylated. ABE indicates the result of the ABE method: +, endogenous protein was palmitoylated; NT, not tested.
| Protein name | CSS-Palm prediction |
Experiments |
||||
|---|---|---|---|---|---|---|
| Highest CSS-Palm score | Position | Sequence | Brain enrichment score | Metabolic | ABE | |
| Postsynapse | ||||||
| TARP γ-2 | 1.913 | 121 | -118GGLCIAA124- | 1.000 | + | NT |
| TARP γ-8 | 2.461 | 144 | -141GGVCCVAA147- | 0.750 | + | + |
| Cornichon-2 | 1.730 | 9 | -6AAFCYML12- | 0.901 | + | NT |
| CaMKIIα | 3.765 | 6 | -3TITCTRF9- | 0.883 | + | + |
| Kalirin7 | 2.297 | 1404 | -1401LLTCCEE1407- | 1.000 | − | NT |
| Homer 1C | 2.687 | 365 | -362LLECS-C | ND | − | NT |
| Neurochondrin | 13.016 | 3 | N-MSCCDL6- | 0.822 | + | + |
| Presynapse | ||||||
| Rab3A | 2.313 | 220 | -217DCAC-C | 0.907 | − | NT |
| Syd-1 | 3.078 | 736 | -733INVC l-C | ND | + | + |
| Liprin-α2 | 4.783 | 3 | N-MMCEVM6- | 0.889 | − | NT |
| Motor protein | ||||||
| KIF5C | 2.183 | 7 | -4PAECSIK10- | 0.935 | − | NT |
| Channels | ||||||
| TRPM8 | 4.234 | 1032 | -1029FKCCCKE1035- | 0.750 | + | NT |
| TRPC1 | 2.547 | 736 | -733KVMCCLV739- | 0.833 | + | NT |
| GPCR | ||||||
| Orexin 2 receptor | 3.500 | 381 | -378AFSCCLG384- | 0.833 | + | NT |
| Focal adhesion | ||||||
| Paxillin | 2.270 | 591 | -588KLFC-C | ND | − | NT |
| Zyxin | 2.012 | 404 | -401CFTCHQC407- | ND | + | NT |
| Tight junction | ||||||
| Par3 | 3.061 | 6 | -3VTVCFGR9- | ND | − | NT |