Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 20;288(27):19882–19899. doi: 10.1074/jbc.M113.473181

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — H3.1, H3.2, and H3.3 are all strongly recruited to the activated transgene array in U2OS cells. A, diagram of the domain structure of the H3 variants showing the locations of the amino acid differences between them. B, Western blot analysis of the YFP-tagged H3 variants using a GFP antibody. γ-Tubulin is used as a loading control. C, localization of H3.1-YFP (a–d) and H3.2-YFP (e–h) at the activated transgene array in relation to the activator, Cherry-tTA-ER. Yellow lines in the enlarged merge insets show the path through which the red and green intensities were measured in the intensity profiles (d and h). Asterisks mark the start of the measured line. Scale bar, 5 μm. Scale bars in enlarged inset, 1 μm. D, percentage of cells in which the histone H3 variants were recruited to the activated transgene array. 100 cells were counted in three independent experiments. S.D. values in the form of error bars are shown in the graph.