TABLE 3.

Catalytic activities of full-length RePC mutants compared with wild type for the ADP phosphorylation reaction using carbamoyl phosphate reaction

Reaction conditions were as follows: 100 mm Tris-HCl (pH 7.8), 25 °C, 7 mm MgCl₂, 3.5 mm MgADP, 0.25 mm acetyl-CoA, 20 mm carbamoyl phosphate.

	kcata	Wild type
	min−1	%
Wild type	11.5 ± 0.3	(100)
Y628F	9.5 ± 0.1	83
Y628A	8.8 ± 0.2	77
D590A	8.2 ± 0.06	71

^a Specific activities were determined in triplicate, and the error reported is the S.D. value of the three determinations.